Composite

Part:BBa_K3349006:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-28)
Revision as of 15:15, 7 October 2020 by Kristiturton (Talk | contribs) (→‎Design Notes)


Pectin Lyase Pnl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for E. coli. The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [4].

Source

Sequence was taken from the Uniprot database for synthesis. The sequence ID from NCBI is KAA8782864.1.

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

2.Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.

3. Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R. and Schwede, T.(2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296-W303.

4. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purification and biochemical properties of a thermostable pectinase from Bacillus haldurans M29. Journal of Molecular Catalysis B: Enzymatic. 94, 77-81