Part:BBa_K3570000

GGPP production enhancement in S. cerevisiae

One of the main goals of our project is to make S. Cerevisiae produce β-carotene. This construction is used in tight synergy with BBa_K3570001. Precisely, β-carotene derives from the mevalonate pathway as do limonene and geraniol (figure 3). We, therefore, decided to enhance the mevalonate pathway and the production of GGPP. According to Rabeharindranto et al. 2019, the enhancement of the mevalonate pathway can be achieved by overexpressing the gene HMG1 and CrtE. Our design differs from this publication in the choice of the promoter. The promoter TDH1 has been chosen instead of Gal1/10 because we planned to use this promoter for the regulation of other genes. We thus created the plasmids containing a truncated version of the HMG1 gene from S. Cerevisiae and the CrtE gene from X. Dendrorhous.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1015
  Illegal PstI site found at 50
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 683
  Illegal NheI site found at 3586
  Illegal NheI site found at 4491
  Illegal NheI site found at 5834
  Illegal PstI site found at 50
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1641
  Illegal BglII site found at 3716
  Illegal BglII site found at 5725
  Illegal BglII site found at 5785
  Illegal BamHI site found at 3376
  Illegal XhoI site found at 705
  Illegal XhoI site found at 3415
  Illegal XhoI site found at 3547
  Illegal XhoI site found at 4366
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1015
  Illegal PstI site found at 50
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1015
  Illegal PstI site found at 50
  Illegal NgoMIV site found at 870
  
• 1000
  COMPATIBLE WITH RFC[1000]