Coding

Part:BBa_K3349004:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)
Revision as of 16:02, 6 October 2020 by Kristiturton (Talk | contribs) (→‎References)


Pectin Lyase PelB-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 261
    Illegal BamHI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus of the GFP reporter.



Source

The pectase sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1. The GFP sequence was taken from uniprot as well (P42212)

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.

2.Roy, A., Kucukural, A., and Zhang, Y. (2010) I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.