Part:BBa_K3610006

EFR from A. thaliana - without signal sequence

This part is the EFR receptor from A. thaliana. It is similar to Part:BBa K3610005 but it lacks the signal sequence and is therefore not in frame. It needs to be expressed with a signal sequence. Either with the native signal sequence or with a signal sequence from another organism.

Usage and Biology

Elongation factor-thermo unstable receptor (EFR) from A. thaliana is a plant pattern-recognition receptor (PRR). It is a cell surface receptor and part of the plants firts defence mechanism against potential pathogens. The EFR receptor is also a leucin-rich-repeats (LRR) receptor-like serine/threonine-protein kinase. The protein consists of an extracellular domain with leucin-rich repeats, a ligand binding domain found in many receptors, a single-pass transmembrane domain and finally an intracellular kinase domain. The ligand binding domain from EFR has high specificity to a bacterial pathogen-associated moleculat pattern (PAMP), namely the epitope elf18 of the abundant protein Elongation Factor Tu (EF-Tu), which is catalyzes the binding of aminoacyl-tRNA (aa-tRNA) to the ribosome in most prokaryotes and therefore is evolutionarily highly conserved. This makes the EFR a receptor that can be activated by the presence of a huge variety of bacteria. Upon binding of the ligand to the extracellular domain, the receptor dimerizes with its coreceptor BRI1-associated receptor kinase (BAK1). This interaction triggers the activation of the intracellular kinase domain of EFR and BAK1, initiating a signal cascade leading to an upregulation of immune response mechanisms.

In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence. We further wanted to see where within the cell the protein localizes.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2683
  Illegal XbaI site found at 363
  Illegal XbaI site found at 2498
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2683
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2683
  Illegal BglII site found at 2348
  Illegal BglII site found at 2376
  Illegal BglII site found at 2919
  Illegal XhoI site found at 2372
  Illegal XhoI site found at 2962
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2683
  Illegal XbaI site found at 363
  Illegal XbaI site found at 2498
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2683
  Illegal XbaI site found at 363
  Illegal XbaI site found at 2498
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  Illegal AgeI site found at 186
  
• 1000
  COMPATIBLE WITH RFC[1000]