Part:BBa_K3417000

ArgK Coding Sequence

This part is the coding sequence of the protein Phaselotoxin Resistant Ornithine Carbamoyltransferase (ROTCase), which substitutes the metabolic role of E. coli ornithine carbamoyltransferase in the bacterial urea cycle. This figure shows the Sequential Bi-Bi Mechanism of ROTCase, in which carbomylphosphate is the first substrate to bind, allowing ornithine to then bind to the complex. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 859
  

Functional Parameters

kcat = 2940 min^(-1)

References

Kelly, J., Rubin, A., Davis, J., Ajo-franklin, C., Cumbers, J., Czar, M., De Mora, K., Glieberman, A., Monie, D., &Endy, D. (2009). Measuring the activity of biobrick promoters using an in vivo reference standard.Journalof Biological Engineering,3(1), 4. https://doi.org/10.1186/1754-1611-3-4

Templeton, M. D., Reinhardt, L. A., Collyer, C. A., Mitchell, R. E., & Cleland, W. W. (2005). Kinetic analysisof the l-ornithine transcarbamoylase from pseudomonas savastanoi pv. phaseolicola that is resistant to thetransition state analogue (r)-n-(n‘-sulfodiaminophosphinyl)-l-ornithine [PMID: 15766270].Biochemistry,44(11), 4408–4415. https://doi.org/10.1021/bi047432x