Composite
Part:BBa_K3634021
Designed by: Laurence Seeley Group: iGEM20_St_Andrews (2020-08-09)
LacI-Controlled CviJI Endonuclease (+ ssrA deg. tag)
The mutant lac promoter PL8-UV5 will regulate the intracellular concentration of R.CviJI through independent lac repressor binding to the operator sites. The ssrA degradation tag AANDENYADAS will prevent unwanted leaky expression of the endonuclease from degrading the plasmid sequences when still required.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 441
Illegal AgeI site found at 584 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |