Coding

Part:BBa_K3634007

Designed by: Laurence Seeley   Group: iGEM20_St_Andrews   (2020-08-05)
Revision as of 15:34, 5 August 2020 by Ls268 (Talk | contribs)

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Heme Oxygenase (ho1) (Optimised for E.coli)

Heme oxygenase (ho1) is the first of two required genes for the conversion of heme into the blue phycobilin phycocyanobilin (PCB), a chromophore required for activation of some two component light-sensing systems (TCS) such as the ccaS/ccaR system in Synechocystis sp. PCC 6803. In the presence of oxygen, heme oxygenase catalyses the opening of the heme ring, releasing iron and generating biliverdin IXα which is then reduced by phycocyanobilin ferredoxin oxidoreductase (pcyA) to produce PCB. PCB can then bind to the appropriate membrane associated histidine kinase of the TCS allowing activation and expression of the output gene in the presence of an activating wavelength of light. The part should be used alongside BBa_K3634006 and BBa_K3634008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 390
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None