Template:TypeIIS/Plasmids/Level2

Revision as of 20:06, 15 June 2020 by Vinoo (Talk | contribs)

When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with BBa_J04455), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.

Registry Name Loop Name Fusion Site 5' MTU Fusion Site 3'
pSB3C01 pEven1 GGAG MTU 1 TACT
pSB3C02 pEven2 TACT MTU 2 AATG
pSB3C03 pEven3 AATG MTU 3 GCTT
pSB3C04 pEven4 GCTT MTU 4 CGCT

Note: Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04455 have been verified.