Template:TypeIIS/Plasmids/Level2
When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with BBa_J04455), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.
| Registry Name | Loop Name | Fusion Site 5' | MTU | Fusion Site 3' |
|---|---|---|---|---|
| pSB3C01 | pEven1 | GGAG | MTU 1 | TACT |
| pSB3C02 | pEven2 | TACT | MTU 2 | AATG |
| pSB3C03 | pEven3 | AATG | MTU 3 | GCTT |
| pSB3C04 | pEven4 | GCTT | MTU 4 | CGCT |
Note:
- The documented sequence shows an additional BsaI site. This would impact assembly out of (but not into) this set of vectors. A new set of vectors should be created or used in the long term.
- Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04455 have been verified.