Plasmid
YCF1pr

Part:BBa_K3600002

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-14)
Revision as of 17:54, 14 June 2020 by Sspoerri (Talk | contribs) (→‎Usage and Biology)


YCF1 promoter part plasmid

This part contains the promoter of the gene YCF1 from S. Cerevisiae.

Usage and Biology

  • activator: Yap1p
  • YCF1 gene encodes ...
  • The promoter TXR2 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to an oxidative stress.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 935
    Illegal EcoRI site found at 1665
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1842
    Illegal SpeI site found at 2810
    Illegal PstI site found at 2819
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Measurements

References

[1]

[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None