Plasmid
HSP12pr

Part:BBa_K3600000:Design

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-09)
Revision as of 15:19, 11 June 2020 by Sspoerri (Talk | contribs) (→‎Design Notes)

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HSP12 promoter part plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 308
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 326
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 308
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 57
    Illegal BsaI.rc site found at 578


Design Notes

The sequence of 500bp preceding the ORF of HSP12 was retrieved from the yeast genome database. No modifications were applied. The presence of 5 stress response elements (STRE) was confirmed by sequence analysis.

Source

Promoter of the small heat-shock protein HSP12 (YFL014W) from the genome of S. Cerevisiae.

References