Generator

Part:BBa_K143073

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)
Revision as of 16:48, 27 October 2008 by Chris (Talk | contribs)

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AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)


AmyE 5' Integration sequence(BBa_K143001) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143052 and BBa_K143064) and the PoPS generator Pveg-gsiB (BBa_K143052).


The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(BBa_K143002) is cloned onto the 3' end of the construct.


The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.


The Pveg-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/Bsubtilis
Parameters
None