Coding

Part:BBa_K3515008:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-25)
Revision as of 13:48, 25 May 2020 by Kody klupt (Talk | contribs)


Potassium binding protein with cysteine modification(s) to bind to a biosensor and FRET to monitor p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 766
    Illegal AgeI site found at 823
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a cysteine for immobilization (A26C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its sequence retrieved from the European Nucleotide Archive (U00096.3) along with our own modifications.

References

Shen, Y., Wu, S.Y., Rancic, V., Aggarwal, A., Qian, Y., Miyashita, S.I., Ballanyi, K., Campbell, R.E. and Dong, M., 2019. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Communications biology, 2(1), pp.1-10.