Composite

Part:BBa_K3042005

Designed by: Rehmat Babar   Group: iGEM19_Georgia_State   (2019-10-11)
Revision as of 03:19, 22 October 2019 by Rbabar2 (Talk | contribs)


Dino III GFP Plasmid

A dinoflagellate optimized reporter plasmid. DinoIII is a dinoflagellate expression system that is constructed on the basis of genomic and transcriptomic data from many dinoflagellates. DinoIII GFP is a dinoflagellate optimized reporter plasmid which was created by the University of Connecticut. This part can be used as a reporter via fluorescence. When the transformed cells fluoresce green, this indicates the expression of the GFP (Specher, 2019). This part has been successfully transformed into O.Marina by UNAM *insert a link to paper here *. iGEM GSU wants to replicate their results with this part as well as into Symbiodinium microadriaticum, another dinoflagellate. ,/img> ,/img> *All sequences/info are from snapgene* Features: RNA elements Sprecher, Brittany & Zhang, Huan & Lin, Senjie. (2019). Nuclear gene transformation in a dinoflagellate. 10.1101/602821.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 682
    Illegal NheI site found at 1781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1201
    Illegal XhoI site found at 1102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1763

AmpR promoter- Ampicillin resistance promoter AmpR- Product: Beta lactamase Confers resistance to ampicillin and related antibiotics

Ori- High copy number ColE1/pMB1/pBR322/pUC origin of replication Cap binding site-Cap binding activates transcription in the presence of cAMP

Lac operator- The lac repressor binds the lac operator to block transcription in E. Coli. This inhibition can be relieved by adding lactose or IPTG Lac promoter- Promoter for E. Coli lac operon Terminator- part k3042003 Cycle 3 GFP- Mammalian codon-optimized cycle 3 GFP (Crameri et al., 1996) Promoter region- part K3042002


[edit]
Categories
Parameters
None