Coding
Part:BBa_K2916010
Designed by: Konstantinos Ragios Group: iGEM19_EPFL (2019-09-25)
Revision as of 03:18, 22 October 2019 by Konstantinos (Talk | contribs)
CK protein equipped with a 6x HIS affinity tag
Creatine kinase is a protein that is used in the OnePot PURE cell-free system.
Usage and Biology
CK participates in aminoacyl-tRNA based protein biosynthesis as a reaction catalysing enzyme.
In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.
For our project we used CK as a part of the protein solution needed for OnePot PURE cell-free system produced with the method of gravity flow affinity chromatography, as described in the protocol we designed.
Characterization
All the data regarding the characterization of that part can be found in the composite part: BBa_K2916045 . Sequence and Features BBa_K2916010
SequenceAndFeatures
Not understood
[edit]
Categories
Parameters
| None |