Part:BBa_K3042002

Promoter Region in Dino III Plasmid

The promoter is a G rich, intergenic region, containing 672 base pairs, between O.Marina rhodopsin tandem repeats found in the DinoIII plasmid. It is incorporated in the DinoIII plasmid to increase expression of arrO, which is a rifampin resistance gene. An increased expression of the rifampin resistance gene as well as a higher cell survival rate was observed. Because no proven promoter exists for dinoflagellates at this time, the “promoter region” was determined by locating the TTTT motif, which is 65 base pairs upstream from the start codon. This motif is present 133 base pairs upstream from the start codon in the DinoIII plasmid, and the value of these sequences cannot be evaluated (Sprecher, 2019).

Sprecher, Brittany & Zhang, Huan & Lin, Senjie. (2019). Nuclear gene transformation in a dinoflagellate. 10.1101/602821.