Terminator

Part:BBa_K2992013

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-09-10)
Revision as of 01:35, 22 October 2019 by JacobGausden (Talk | contribs) (→‎Characterisation)

Usage

Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrKDE operon of C. sporogenes, following chromosomal knock-in of botR in part BBa_K2992025, at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).

Terminator.png

Tfad from C. tetani

  • Transcriptional terminator sequence from the FAD-dependent oxidoreductase, fad, from Clostridium tetani

Secondary Structure

Mfold-K2992013-1.png

Characterisation

This basic part was used upstream of PbotR to prevent chromosomal read-through from the pyrKDE operon, upon integration of the PbotR-botR module at this locus. See our results page for more information.

References

Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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