Other

Part:BBa_K3117037

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-18)
Revision as of 01:33, 22 October 2019 by DSchreyer (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


SpyTag (E.coli expression) The SpyTag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins. It is used in the construct to recognize the corresponding molecule, Catcher, and form a covalent isopeptide bond between the side chains of an reactive aspartate and lysine in SpyCatcher (Hatlem et al. 2019).


Usage and Biology

The SpyTag/SpyCatcher system is based on a modified domain from a streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag), which can bind spontaneously and form an isopeptide bond (Hatlem et al. 2019).

References

1. Hatlem, Daniel; Trunk, Thomas; Linke, Dirk; Leo, Jack C. (2019): Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. In: International journal of molecular sciences 20 (9). DOI: 10.3390/ijms20092129.

[edit]
Categories
Parameters
None