Coding

Part:BBa_K3317002

Designed by: Erick Hernandez, Abigail Torres and Shakti Delgadillo   Group: iGEM19_UANL   (2019-10-15)
Revision as of 23:32, 21 October 2019 by Erick419 (Talk | contribs)


Protocatechuate decarboxylase (aroY) from Klebsiella pneumonia subsp.

Protocatechuate decarboxylase (aroY gene) converted the protocatechuic acid into catechol. The enzyme was modified with a signal peptide at its N-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et. al 2017) This is a hexameric enzyme



Wild type enzyme information: E. C. 4.1.1.63

Km 0.59

Kcat 9x10-5 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1045


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Parameters
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