Part:BBa_K2995000
Paph promoter
Constitutive promoter for expression in E. coli and B. japonicum.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
(Waterloo iGEM 2019)
To characterize our promoter we cloned it upstream of the RBS in BBa_E0240 (RBS-GFP-Term) and compared it's GFP expression with the already well characterized BBa_J23101 promoter in BBa_I20260. To limit the impact of other variables we insured that BBa_I20260 and BBa_E0240 shared the same backbone, RBS and terminator. In addition to characterizing this promoter with respect toBBa_I20260 we also confirmed proper function of this promoter in the pRJPaph-bjGFP plasmid which was used for expression in Bradyrhizobium for our 2019 project.
Note:
empty DH5alpha was used as a negative control for GFP expression
1. Cell were grown overnight in LB media at 37 degrees
2. The following morning GFP expression was quantified using the Synergy 4 BioTek plate reader.
The graph below indicates the results from this characterization experiment:
(Excitation: 485, Emmission: 525)
| None |