Composite

Part:BBa_K3320005

Designed by: Isaac de Paula Guerreiro   Group: iGEM19_Amazonas-Brazil   (2019-10-16)
Revision as of 23:20, 21 October 2019 by Jocomodaro (Talk | contribs)


Basic Repressor Unit

This composite part is the basic constitution of our AND gate, repressing pTet with TetR protein regulated by phiF promoter.

Usage and Biology

pPhlF is an orthogonal promoter repressed by PhlF Repressor. In the absence of PhlF repressor, produced by the lactate promoter, this part is constitutively expressing TetR, which represses the production of BBa_K3320013.

Assembly

This part was cloned on pUC19 vector, totaling 4587 bp.

BBa K3320005 Map.png

Figure 1. BBa_K332005 plasmidial map.

BRUGel.jpeg

Figure 2. BBa_K332005 confirmation of cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1596


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Categories
Parameters
None