Part:BBa_K3284002:Design
Carcinoembryonic antigen (CEA) with His tag
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 40
Illegal NotI site found at 354
Illegal NotI site found at 2137 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 532
Illegal AgeI site found at 1618 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Tumor markers are molecules occurring in blood or tissue that are produced by a tumor associated with a cancer or by the host in response to the cancer. Measurement or identification of tumor markers is useful for clinical diagnosis or patient management. Carcinoembryonic antigen (CEA), the first oncofetal antigens, consists of a large family of cell surface glycoprotein (molecular weight: 150–300 kDa). It is associated with plasma membrane of tumor cells, from which it may be released into the blood. In lung cancer serological tests, one of the most commonly used circulating markers is CEA and CYFRA. But our team chose CEA as our main target to be diagnosed, because CEA is proven to have a high sensitivity and specificity other than tumor markers.
In this project, CEAker is a synthetically combined receptor used as the diagnostic tool system. TGFBR1, which is originally found in human body, directly interacts with Carcinoembryonic Antigen (CEA). CEA itself functions as an intercellular adhesion molecule and is upregulated in a wide variety of human cancers, including lung cancer. CEA-binding domain mainly lodged in TGFBR1’s extracellular environment.
We added His tag in order to synthesize pure CEA (our receptor ligand).
Source
CEACAM5.