Part:BBa_K3183002

Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp.

P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

This is a constitutive promoter from the lactate dehydrogenase (ldhL) promoter from Lactobacillus acidophilus¹. It has been subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363².

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Use by Team Oxford 2019

The ldhL promoter is a constitutive promoter that has been used in Lactobacillus reuteri in our composite part BBa_K3183100.

Characterisation

The part was characterised in the composite part BBa_K3183101.

References

1. Kim, S F et al. “Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene.” Applied and environmental microbiology vol. 57,8 (1991): 2413-7.
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.