Coding

Part:BBa_K3208002

Designed by: Slavil Peykov   Group: iGEM19_Bulgaria   (2019-10-21)
Revision as of 21:01, 21 October 2019 by Slavil (Talk | contribs)


Expression module that produces Carcharin_2

This part is an expression module that produces Carcharin_2. Carcharin_2 is a putative antimicrobial peptide found in the genome of the great white shark.

Charcharin_2: SRSSRAGLQLPAGRVHRLLRK


The expression module contains:

- T7 promoter (2-20)

- strong RBS (44-66)

- start codon ATG (74-76)

- Charcharin_2's CDS (77-139)

- stop codon (140-142)

- T7 terminator (150-197).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 21


[edit]
Categories
Parameters
None