Coding

Part:BBa_K3042000

Designed by: Rehmat Babar   Group: iGEM19_Georgia_State   (2019-10-11)
Revision as of 21:01, 21 October 2019 by Ldasneves1 (Talk | contribs)


Dinoflagellate Codon Optimized RFP

This part is a codon-optimized version of RFP for use in use in Dinoflagellates, a single-celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector. The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have many codons that code for the same amino acids, favorable codons of the dinoflagellates were chosen using the chart below to ensure the translation of the red fluorescent protein. This part can be used as a reporter or indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.

[Insert chart picture here]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 696
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None