Coding

Part:BBa_K3110000:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-13)
Revision as of 20:53, 21 October 2019 by Dibyasaha (Talk | contribs) (→‎Design Notes)

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lldD (L-Lactate Dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 586


Design Notes

We took the sequence that is present in wild type E.coli (from ensemble) and codon optimized it using Twist Bioscience codon optimized tool to make it synthesis compatible and remove an in between EcoRI site(as we used this enzyme in cloning purpose).

Source

The sequence was synthesized by Twist Biosciences.

References