Composite

Part:BBa_K3015011

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-16)
Revision as of 20:11, 21 October 2019 by Vanessap71 (Talk | contribs)


Riboswitch Sensor cassette

This genetic device was used to proof the functionality of a Riboswitch-based diagnostic method.

Usage and Biology

This is the genetic device we realized in the lab with a transcriptional Theophylline-Riboswitch. T7-Polymerase will be expressed in the Presence of Theophylline, which will further induce the strong expression of amilCP, a blue chromoprotein, that acts as visual readout. The designed and constructed system can easily be adapted for detection of various disease-causing agents by just changing the riboswitch-sequence according to the target.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2886
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2820
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2886
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2886
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None