Generator

Part:BBa_K602008

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-05)
Revision as of 18:34, 21 October 2019 by Youma (Talk | contribs) (→‎Team Botchan_Lab_Tokyo 2019 characterization)

D. radiodurans RecA expression device

D. radiodurans RecA with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses RecA. Should increase DNA damage repair capability and radioresistance of host E. coli.

Please see Experience page for characterization details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


Team Botchan_Lab_Tokyo 2019 characterization

We characterized BBa_K602008.

description

It is RecA (composite parts) that combined the psB1C3 is vector in the basic parts with RecA is the coding parts in an in-fusion cloning. The graph below shows the growth curves of competent cells, DH5α without this plasmid and the composite parts.

T--Botchan Lab Tokyo--recAgraph.pdf

This chart is standard deviation (SD) of the technical replicate, and the SD is calculated based on data that originally follow a normal distribution. Therefore, it is necessary to determine whether the data follow a normal distribution by the Shapiro-Wilk test. However, the SD was calculated assuming that all data follow a normal distribution because the number of samples worthy of the Shapiro Wilk test cannot be shake-cultured due to laboratory problems.

conclusion

Competent cells grew as expected.

[edit]
Categories
Parameters
None