DNA

Part:BBa_K2919000:Experience

Designed by: Evan Harrison   Group: iGEM19_MichiganState   (2019-10-04)
Revision as of 16:36, 21 October 2019 by Harr1335 (Talk | contribs) (→‎Applications of BBa_K2919000)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2919000

This part was used in an attempt to make a toxin-antitoxin saftey system that would limit horizontal gene transfer. The part was used with a cumate inducible, CuO, promoter to prevent the toxin to kill the cell immediately. When induced with sufficient cumate the toxin worked to prevent cell growth in E. coli.


Cumate ghoT growthcurve.png This graph show that the toxin, ghoT, works by showing that the E. coli with the toxin insert when induced by cumate had lower OD600s than the E. coli without the toxin insert.

User Reviews

UNIQ5a8568ddd828cf8f-partinfo-00000000-QINU UNIQ5a8568ddd828cf8f-partinfo-00000001-QINU