Reporter

Part:BBa_K3137004

Designed by: Jiamin Chen   Group: iGEM19_JiangnanU_China   (2019-10-07)
Revision as of 08:48, 21 October 2019 by LWJ (Talk | contribs)


mCherry

The mCherry can express red fluorescent protein and it is used as a marker gene in the process of reporting the number of cells.

Usage and Biology

When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF(BBa_K3137003) will induce expression of the red fluorescent protein gene mCherry, which will make the bacteria display red fluorescence.

Characterization of PglcF

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None