Device
Part:BBa_K112019
Designed by: Bing Xia Group: iGEM08_UC_Berkeley (2008-10-24)
Lambda phage lysis device
Lysozyme and holin production are driven by pBad, and antiholin is driven by PconC5.
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 33
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 33
Illegal NheI site found at 1184
Illegal NheI site found at 2230
Illegal NheI site found at 2253 - 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 33
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 33
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 33
Illegal NgoMIV site found at 2616 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1050
[edit]
Categories
Parameters
//function/celldeath
None |