Part:BBa_K3031015:Design

LuxI with FUR box located in the -10 region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.

<img src="T--SUIS_Shanghai--ironQSassay.png" alt="IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)" style="width:128px;height:128px;">

Source

BioBrick sequences.

References

Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.

Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.