Regulatory

Part:BBa_K3031015:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-15)
Revision as of 06:03, 21 October 2019 by Davystar (Talk | contribs)


LuxI with FUR box located in the -10 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments. <img src="T--SUIS_Shanghai--ironQSconstructs.png" class="scale scale1" /> <img src="T--SUIS_Shanghai--ironQSassay.png" class="scale scale1" /> 

                               <img class="expoptionimg" src="T--SUIS_Shanghai--ironQSconstructs.png">

Source

BioBrick sequences.

References

Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.

Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.