Reporter

Part:BBa_K079031

Designed by: Giovanni Mariotta   Group: iGEM08_Bologna   (2008-10-24)
Revision as of 20:29, 21 October 2009 by Francesca ceroni (Talk | contribs)

GFP reporter protein under the control of the BBa_J23118 promoter

GFP reporter protein under the control of the BBa_J23118 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705

We experimentally observed that bacterial growth curve can change if cells are transformed with different genetic circuits. Moreover, we reasoned that is not sufficient to characterized a part or a device in terms of fluorescence, but is also important to understand how the bacterial cells grow and how the fluorescence expressed is related to the growth phase. For this reason, we analized both the growth and fluorescence curves in time and used them to experimentally characterize our parts and fill this Registry experience section.



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