Coding

Part:BBa_K2960003

Designed by: Michelle Croen   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 01:34, 21 October 2019 by Aemetzloff (Talk | contribs)


mlrC with TorA tat Sequence

This is a composite part consisting of a constitutively-active promoter sequence (J23100), a ribosome binding site (BBa_J61100), the mlrC gene (BBa_k2960008), the TorA tat sequence (BBa_K1012002), a 3x FLAG tag (BBa_K823034), and a terminator (BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
    Illegal BglII site found at 932
    Illegal BamHI site found at 1467
    Illegal XhoI site found at 1211
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None