Coding

Part:BBa_K108007:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)
Revision as of 05:18, 25 October 2008 by Angel6767Q (Talk | contribs) (→‎Source)

FliD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

FliD was cloned from E.coli K12 DH10B genome (reference 1).

References