Regulatory

Part:BBa_K3292005

Designed by: Gerardo David Díaz Renovato, Abril Diosdado Hernández   Group: iGEM19_Tec-Monterrey   (2019-10-20)
Revision as of 20:35, 20 October 2019 by AbrilDH (Talk | contribs)


DNA synthesis regulated promoter (yorB)

This promoter regulates the expression of the YorB protein in B. subtilis subtilis str. 168 and was initally reported by Hutter et al. (2004) and further characterized by Urban et al. (2007) for its induction by antibiotics that interference with DNA biosynthesis in B. subtilis.

As part of our project, we flanked the core sequence with sites of type IIs restriction enzymes, particularly BsaI and BsmBI (also called Esp3I), to allow its usage with Golden Gate cloning. Finally, we added biobrick prefix and suffix to make it compatible with RFC 10 standard, and M13 forward and reverse primer sequences to enable the PCR amplification of this parts as gBlocks.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None