Composite

Part:BBa_K3286011

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 21:22, 21 October 2019 by AlexNiederau (Talk | contribs)

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sgRNA expression cassette (double target)

Double expression module of the sgRNA for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer.

For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109 and the other under control of the constitutive Tac promoter.

Anderson promoter J23109: Part:BBa_J23119

Tac promoter: Part:BBa_K2933026

sgRNA expression cassette: Part:BBa_K3286001

rho-independent terminator: Part:BBa_K3286002


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None