Part:BBa_K3127996
This part contains LasI ，LasR ，CcaR，CcaS，ho1，pcyA and ccdB.
It is a part of the Light Controlled Bacteria Co-culture system.This part contains LasI ，LasR ，CcaR，CcaS，ho1，pcyA and ccdB. When receiving the green light, this part can release 30C6HSL and produce CcdB protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 658
Illegal NheI site found at 715
Illegal NheI site found at 1577
Illegal NheI site found at 1600
Illegal NheI site found at 3388 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2007
Illegal XhoI site found at 1431 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3089
Illegal NgoMIV site found at 3749
Illegal NgoMIV site found at 5490
Illegal AgeI site found at 3946 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6358
Characterization
Figure 1/2 shows the trends of each bacteria in the LCBC-system. We got the data through the enzyme-labelled instrument and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. There are two kinds of E.coli. One kind of E.coli carried sfGFP on the genome. The other kind of E.coli carried RFP on the genome. Cells were individually cultured in the LB medium for 12 hours before common inoculated in 12-well plates in LB medium in the first 13 hours. Figure 1 shows the different fluorescent intensity of E.coli under the light of 640-650 nm wavelength for the first 13 hours and the light of 460-465 nm wavelength for the next 12 hours.Figure2 shows the different fluorescent intensity of E.coli under the light of 640-650 nm wavelength for the first 13 hours and the light of 530-535 nm wavelength for the next 12 hours.
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