Composite

Part:BBa_K3196034

Designed by: XinRan Rao   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 13:51, 20 October 2019 by GlacierHOLE (Talk | contribs)

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Pgpda-GFP

Pgpda is a promoter amplified from the genome of Aspergillus niger, which can achieve maximum response at around pH5.0 and express the corresponding coding sequence. We can test the function of the promoter by measuring the fluorescence intensity at different pH gradients and get the related parameters. After testing the promoter, replace the GFP protein with the target gene.

Usage and Biology

We cultured Pichia pastoris containing PgpdA-gfp in pH=4/5/6 medium for 4 hours, and then the fluorescence of each tube was observed under UV lamp. The results showed that it had better expression effect under the condition of pH=5:

Figure1.pH5 shows higher fluorescence intensity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 207
    Illegal BamHI site found at 850
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None