Part:BBa_K3196028
Pgas
promoter
Characterization
Pgas, a low-pH-inducible promoter, promoted gene expression efficiently at low pHs, such as pH 2.0.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 51
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional parameters (Added by XJTU 2021)
As is shown in Fig.1 (Yin et al., 2017), the strength of Pgas is mainly dependent on the pH, while limited influenced by the types of acids.
Fig. 1 Factors affecting relative Pgas-inducing capacity. (A) Influence of acid type on Pgas-inducing capacity. (B) Influence of citrate concentration on Pgas-inducing capacity. (C) Fold changes in CAD expression at different pHs. Error bars represent the standard errors of the means of results for three biological replicates.
The author gives an equation with linear regression analysis to describe the relationship between the relative expression level and pH: log(E) = [(6.042/pH) − 0.9059] in which, E represents the relative expression level compared with expression at pH 7.0. The correlation coefficient factor (R2) is 0.9962.
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