Part:BBa_K3196016

AOX1-Kozak-FLO10-pelA-His tag-AOX1 Terminator

The FLO10 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. pelA can synthetic pectin lyase.

Characterization

This is a four section for degrade and transfer pectin part.

Figure1. T--HUST--China--2019-FLO10pelA

DNA Gel Electrophoretic

After we link FLO10 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1787
  Illegal BamHI site found at 937
  Illegal BamHI site found at 948
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]