Part:BBa_K3196033

FLO10-αpro-Geneart Laccase

This part is an improvement of part Baa_K500002, which codes the Geneart Laccase. Geneart Laccase is a polyphenol oxidase, which belongs to the family of blue multicopper oxidases. Laccases oxidize a broad range of substrates, preferably phenolic compounds.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 349
  Illegal BglII site found at 886
  Illegal BglII site found at 1582
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

This part is an improvement of part Baa_K500002, which codes the Geneart Laccase. Geneart Laccase is a polyphenol oxidase, which belongs to the family of blue multicopper oxidases. Laccases oxidize a broad range of substrates, preferably phenolic compounds. Compering with part BBa_K500002, We add a productive signal peptide called FLO10-αpro before the gene.

Experiments

We insert the segment into pPIC9K plasmid.

Figure1:This is the construct process of the new part and the old part into pPIC9k plasmid.

By using BamHⅠ and SnaBⅠ,we make a kick on the FLO10-pPIC9k and replace the FLO10 signal peptide with BBa_K500002 fragment(the fragment is amplified by PCR). By using SnaBⅠand EcoRⅠ, we add BBa_K500002 fragment after FLO10 signal peptide.

Here are the DNA gel electrophoresis result:

Figure2:The construction is successful.