Plasmid

Part:BBa_K3179103:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-16)
Revision as of 03:52, 20 October 2019 by Bigxushen (Talk | contribs) (→‎Design Notes)

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pUbC-rtTA3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 999
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 999
    Illegal NgoMIV site found at 473
    Illegal AgeI site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists of promoter pUbC and basic part rtA3 in vector pAVV-UbC. pUbC from human ubiquitin C gene and is a mammalian promoter. pUbC is usually used for regular expression and has a lower expression. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.

Source

pUbC-rtTA3

References

1 Schorpp M, Jäger R, Schellander K, et al. The human ubiquitin C promoter directs high ubiquitous expression of transgenes in mice[J]. Nucleic acids research, 1996, 24(9): 1787-1788. 2 Xie Z, Wroblewska L, Prochazka L, et al. Multi-input RNAi-based logic circuit for identification of specific cancer cells[J]. Science, 2011, 333(6047): 1307-1311.