Composite

Part:BBa_K3009021:Design

Designed by: Carolin Ruckes   Group: iGEM19_Freiburg   (2019-10-16)
Revision as of 13:50, 21 October 2019 by NGensch (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


FPR2-receptor with mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1063
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For receptor expression we wanted to make sure that it would be functionally active to conduct our signalling studies. For this the surface expression of FPR2 was paramount for this there is an mtlight1 signal sequence present which will direct the receptor to the plasmamenbrane. Furthermore we needed to make sure that the GPF fusion didn’t interfere with the signalling mechanisms of FPR2. Our decision to add GFP to the C-terminus of the FPR2 proved to be the right one since it retained its functionality.



Source

Homo sapiens

References

[1] Gordon Y. C. Cheung, Dorothee Kretschmer, Shu Y. Queck,Hwang-Soo Joo, Rong Wang, Anthony C. uong, Thuan H. Nguyen, Thanh-Huy L. Bach,Adeline R. Porter, Frank R. DeLeo, Andreas Peschel, and Michael Otto (2014) Insight into structure-function relationship in phenol-solublemodulins using an alanine screen of the phenol-solublemodulin (PSM)ɑ3 peptide. The FASEB Journal, 153-161

[2] Kretschmer, D., Gleske, A. K., Rautenberg, M., Wang, R.,Koberle, M., Bohn, E., Schoneberg, T., Rabiet, M. J., Boulay, F.,Klebanoff, S. J., van Kessel, K. A., van Strijp, J. A., Otto, M., andPeschel, A. (2010) Human formyl peptide receptor 2 senseshighly pathogenicStaphylococcus aureus. Cell Host Microbe7,463–473