Coding
AID

Part:BBa_K103001

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-09-14)
Revision as of 11:01, 24 October 2008 by MKrzyszton (Talk | contribs)

AID protein

AID protein

Usage and Biology

  • AID (Activation Induced Deamidase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
  • We tried to use AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 78
    Illegal SapI site found at 179


[edit]
Categories
//cds/enzyme
Parameters
None