Part:BBa_K2957007

hEF1a, IL-8 (CXCL-8), NeonGreen Tag

Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1256
  Illegal PstI site found at 699
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 699
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 473
  Illegal BamHI site found at 715
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1256
  Illegal PstI site found at 699
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1256
  Illegal PstI site found at 699
  Illegal AgeI site found at 81
  Illegal AgeI site found at 1262
  
• 1000
  COMPATIBLE WITH RFC[1000]