Composite
Part:BBa_K2957031
Designed by: Melody Wu Group: iGEM19_MIT (2019-10-19)
TRE, CCL5
Contents: TRE promoter with CCL5 Gene, Carb resistance
What is it?: A Composite part; we call it a pL1
What does it do?: It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells.
How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. We tested it with Jurkats. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2943
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2943
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2943
Illegal NgoMIV site found at 1479
Illegal AgeI site found at 2949 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2623
Illegal BsaI.rc site found at 2921
Illegal SapI site found at 402
Illegal SapI.rc site found at 2560
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Categories
Parameters
None |