Composite

Part:BBa_K2996008

Designed by: Yunqing Wang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-04)
Revision as of 22:04, 19 October 2019 by Pedestal (Talk | contribs)


pLuxR upstream of cas1/2

The expresssion of cas1-cas2 complex can be triggered by the addition of AHL.

  1. Usage and Biology

Expression of the CRISPR adaptation proteins Cas1 and Cas2 promotes unidirectional integration of 33–base pair DNA spacers into our designed RSRL array.

On the other hand, the input information is now acyl-homoserine lactones. In the transcription activation gene circuit, luxI can be put downstream of promotor J23001, enabling the expression of Luxl enzyme when dcas9 binds to lure sequence. AHL molecule production will then activate the expression of cas1-cas2 complex, in this way, the off-target incidence can be recorded.


  1. Result

蛋白胶图

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None