Coding

Part:BBa_K3105667

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-09-24)
Revision as of 01:13, 21 October 2019 by JonasG (Talk | contribs)

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Aryl-alcohol oxidase

Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris

In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).

AAO facilitates following reaction:

primary aromatic alcohol + O2 = aromatic aldehyde + H2O2


References:
Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
    Illegal BamHI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None